《植物生理学报》 2016, 52(8): 1207-1213

异源表达牡丹PsSPL基因影响拟南芥营养生长与开花时间

王艳艳, 管世铭, 盖树鹏, 刘春英, 战新梅, 张玉喜^*

青岛农业大学生命科学学院/山东省高校植物生物技术重点实验室, 山东青岛266109

通信作者：张玉喜；E-mail: zhang-yuxi@163.com

摘　要：

课题组前期已经获得了牡丹(Paeonia suffruticosa) SQUAMOSA启动子结合蛋白基因(PsSPL)的全长cDNA, 本研究在此基础上, 实时定量PCR (quantitative real-time PCR, qPCR)分析PsSPL基因在初花期牡丹的营养器官根、茎、叶和花器官花瓣、萼片、雄蕊中的表达特性, 结果表明PsSPL基因在根中表达水平最高, 在茎、花瓣和叶中次之, 在雄蕊和萼片中表达水平最低。本文构建了牡丹PsSPL基因的超表达载体并在野生型拟南芥中异源表达, 分析鉴定了3个转基因株系。通过表型观察分析, 发现异源表达PsSPL基因的拟南芥开花时间较野生型提前了约3 d, 莲座叶减少了2~3片。PCR结果表明PsSPL基因通过促进LFY基因从而促进开花提前, 同时发育早期的根长增长, 营养生长结束后株高增高。可见, PsSPL基因的功能不是单一的, 它在植株营养生长和生殖生长等方面都发挥了重要作用。

关键词：PsSPL; 表达模式; 异源表达; 开花时间; 营养生长

收稿：2016-03-24   修定：2016-07-26

资助：国家自然科学基金(31471908和31372104)。

Heterologous expression of tree peony (Paeonia suffruticosa) SQUAMOSA promoter-binding protein-like (PsSPL) gene in Arabidopsis thaliana affects the vegetative growth and flowering time

WANG Yan-Yan, GUAN Shi-Ming, GAI Shu-Peng, LIU Chun-Ying, ZHAN Xin-Mei, ZHANG Yu-Xi^*

College of Life Sciences, Qingdao Agricultural University / Key Lab of Plant Biotechnology in Universities of Shandong Province, Qingdao, Shandong 266109, China

Corresponding author: ZHANG Yu-Xi; E-mail: zhang-yuxi@163.com

Abstract:

In our recent research, the full-length cDNA sequence of PsSPL have been obtained. In this study, the expression pattern of PsSPL at the early stage of flowering was analyzed by quantitative real-time PCR (qPCR), and the results show that PsSPL was expressed ubiquitously in various organs and tissues, but the expression levels were different, and the transcript of PsSPL was the highest in the root, lower in stem, petal and leaf, and the lowest in the stamen and sepal. In addition, the over-expression vector of PsSPL gene was constructed and heterologously expressed in wild Arabidopsis thaliana. Three transgenic lines were identified. By phenotypic analysis, heterologous expression of the PsSPL gene in A. thaliana accelerated early flowering for about 3 days and reduced the number of rosette leaves (2~3). The results of PCR indicate that PsSPL gene accelerated flowering by promoting the expression of FLY gene. In addition, heterologous expression of the PsSPL gene in A. thaliana promoted main root length at the early stage of growth and increased plant height after completing vegetative growth. Therefore, the PsSPL gene is not a single functional gene, and it plays important roles both in vegetative and reproductive growths.

Key words: PsSPL; expression pattern; heterologous expression; flowering time; vegetative growth